Residue microbiotas during BSFL bioconversion under the independent s... - SRA

SRX24123690: Residue microbiotas during BSFL bioconversion under the independent strategy
1 ILLUMINA (Illumina NovaSeq X) run: 65,490 spots, 27.6M bases, 11.6Mb downloads

Design: The total DNA of all samples (including initial waste materials, residue samples, and larval gut samples) was extracted using the DNeasy PowerSoil Kit (PowerSoil, QIAGEN, Hilden, Germany) according to the manufacturers instructions. Once extracted, the DNA sample was tested by 1% agarose gel electrophoresis. We used the general bacterial primer pair 515F (5-ACTCCTACG GGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) to amplify the V4 hypervariable region of the bacterial 16S rRNA gene. PCR was used to amplify the intact DNA samples and then the PCR products were detected by 2% agarose gel electrophoresis. Use the AxyPrep DNA Gel Extraction Kit (AXYGEN Co. Ltd.) to isolate the PCR products. Illumina Novaseq PE250 platform was selected for double-end sequencing with an average of 50,007 raw reads. The raw data of high-through sequencing were available at the NCBI Sequence Read Archive (SRA) database under the accession.

Submitted by: Zhejiang University

Study: Residue and larval gut microbiotas during BSFL bioconversion under two strategies

PRJNA1093236 • SRP499176 • All experiments • All runs

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This study combined a laboratory-scale experiment and a case study to explore the BSFL bioconversion of agri-food waste (chicken manure and food waste) generated in Gaoqiao Subdistrict, China under the independent and co-treatment strategies.

Sample: not provided

SAMN40649936 • SRS20909218 • All experiments • All runs

Organism: gut metagenome

Library:

Name: CM4_r2

Instrument: Illumina NovaSeq X

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Runs: 1 run, 65,490 spots, 27.6M bases, 11.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR28522783	65,490	27.6M	11.6Mb	2024-04-06

ID:: 32434192

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